05/01/18 - 9:45 AM to 11:00 AM
Department Seminar: Professor Vicki Hopper Wysocki
"Native MS in structural biology: surface collisions of protein complexes"
Characterization of the overall topology and inter-subunit contacts of protein complexes, and their assembly/disassembly and unfolding pathways, is critical because protein complexes regulate key biological processes, including processes important in understanding and controlling disease. Conventional structural biology methods such as X-ray crystallography, cryoEM, and nuclear magnetic resonance provide high-resolution information on the structures of protein complexes. However, other biophysical methods that provide structural data (e.g. stoichiometry and subunit connectivity) on protein complexes are also important. Native mass spectrometry is an approach that provides critical structural information with higher throughput on low sample amounts. The power of native MS increases when coupled to ion mobility (IM-MS), a technique that measures rotationally averaged collisional cross sections and thus direct information on conformational changes, or to high resolution mass spectrometry (HRMS). This presentation illustrates surface-induced dissociation/ion mobility SID/IM MS and SID HRMS for characterization of topology, intersubunit connectivity, and other structural features of multimeric protein complexes. Data for a number of protein-partner complexes are under investigation, where the partner can be small molecule ligand, protein, DNA, or RNA.
Professor Wysocki is the Ohio Eminent Scholar of Macromolecular Structure and Function, and director of the Campus Chemical Instrument Center in Department of Chemistry and Biochemistry at Ohio State University. She earned her bachelor's degree in chemistry at Western Kentucky University in 1982, and her doctorate in chemistry at Purdue University in 1987. After doing post-doctoral work at Purdue and at the Naval Research Laboratory, she joined Virginia Commonwealth University as an assistant professor in 1990. She was promoted to associate professor in 1994. She joined the University of Arizona in 1996, and was promoted to professor in 2000. Most recently, she was chair of the Department of Chemistry and Biochemistry at Arizona. Professor Wysocki joined OSU in August 2012, as an Ohio Eminent Scholar.
Research in Professor Wysocki's group is separated into three broad areas:
- determination of peptide dissociation mechanisms as a means for improving programs used for automated sequencing of peptides and proteins,
- biomarker discovery for foreign organism detection, and disease diagnosis using proteomics methods, and
- implementation of surface-induced dissociation into commercial time-of-flight instruments.