10/07/19 - 4:00 PM to 5:00 PM
Kolthoff Lectureship #1: Professor Graham Cooks
Izaak M. Kolthoff Lectureship in Chemistry
Mass Spectrometry at Work: Diagnostics and Beyond
This presentation reviews methods of ionization emphasizing desorption and spray ionization methods, and focusing on the ambient method that combines desorption with spray, viz. desorption electrospray ionization or DESI. The “at work” of the title is illustrated with example of drugs in biofluids, contaminants in food, but most particularly, in the use of DESI-MS for intraoperative brain cancer diagnostics. This is done rapidly, in the operating room, by analyzing tissue biopsy smears for diagnostic lipids and oncometabolites. The data are indicative of tumor type (e.g. glioma) and the extent of infiltration at the tumor margins. It will be shown that isocitrate dehydrogenase (IDH) mutation can be determined, intraoperatively by DESI, and that this offers new tumor management options that may increase survival by impacting the extent of surgical resection. References:
 Cooks, et al. Science, 311 (2006) 1566-1570
 Pirro et al., PNAS, 114 (2017) 6700-6705
 Pu et al. Anal. Bioanal. Chem. 411 (2019) 1508 -1508
Photo credit: John Underwood, Purdue University
Graham Cooks was educated at the University of Natal and at Cambridge University and is the Henry Bohn Hass Distinguished Professor of Chemistry at Purdue University. His interests in mass spectrometry include ion soft landing at surfaces, the kinetic method of thermochemical measurement, and high throughput and on-line reaction monitoring. Several new types of mass spectrometers have been constructed in his laboratory, including miniature and hybrid instruments. He contributed to the development of desorption ionization and tandem mass spectrometry, and to ambient mass spectrometry especially desorption electrospray ionization and its applications to medical diagnostics. Professor Cooks has authored more than 1,200 publications, served as PhD thesis adviser to 138 students, has an h-index of 104 [ISI Web of Science], and is a member of the National Academy of Sciences and the American Academy of Arts and Sciences.
Cooks’ research work is focused on mass spectrometry. He was a pioneer in the conception and implementation of tandem mass spectrometry (MS/MS) and of desorption ionization, especially molecular secondary ionization mass spectrometry (SIMS). His group now focusses on miniature portable mass spectrometers using ambient ionization, and applies this combination to problems of trace chemical analysis at point-of-care. His work on ionization methods led to the ambient method of desorption electrospray ionization (DESI) the applications of which include tissue diagnostics during cancer surgery. His interests in the fundamentals of ion chemistry focus on chiral analysis based on the kinetics of cluster ion fragmentation. His group also studies collisions of ions at surfaces. This experiment aims at new methods of molecular surface tailoring and analysis, and on nanomaterials preparation by soft-landing of ions and charged droplets. A form of preparative mass spectrometry based on accelerated reactions in microdroplets is being developed.
Kolthoff Lectureship in Chemistry
Izaak Maurits Kolthoff was born on February 11, 1894, in Almelo, Holland. He died on March 4, 1993, in St. Paul, Minnesota. In 1911, he entered the University of Utrecht, Holland. He published his first paper on acid titrations in 1915. On the basis of his world-renowned reputation, he was invited to join the faculty of the University of Minnesota’s Department of Chemistry in 1927. By the time of his retirement from the University in 1962, he had published approximately 800 papers. He continued to publish approximately 150 more papers until his health failed. His research, covering approximately a dozen areas of chemistry, was recognized by many medals and memberships in learned societies throughout the world, including the National Academy of Sciences and the Nichols Medal of the American Chemical Society. Best known to the general public is his work on synthetic rubber. During World War II, the government established a comprehensive research program at major industrial companies and several universities, including Minnesota. Kolthoff quickly assembled a large research group and made major contributions to the program. Many of Kolthoff’s graduate students went on to successful careers in industry and academic life and, in turn, trained many more. In 1982, it was estimated that approximately 1,100 Ph.D. holders could trace their scientific roots to Kolthoff. When the American Chemical Society inaugurated an award for excellence in 1983, he was the first recipient.