04/01/20 - 4:00 PM to 5:00 PM
Special Seminar: Professor Dan Fu
Quantitative chemical imaging of structure and function in living biological systems – from single cells to animals
Wednesday, April 1, 4pm CT, https://umn.zoom.us/j/166638881
Cell heterogeneity plays a critical role in many pathophysiological processes such as cancer development and neurodegeneration. However, phenotypic variations of individual cells in a complex organ are often intractable by traditional analytical techniques. The main obstacles are the limited amount of analyte in a single cell and the need for noninvasive in situ analysis in order to preserve cell function and microenvironmental information. My lab focuses on the development of label-free pump probe microscopy techniques that enables quantitative chemical and functional measurements at high spatial and temporal resolution from cultured cells to living animals. Specifically, we apply broadband and high sensitivity stimulated Raman scattering microscopy, an emerging chemical imaging tool, to study cell organization, cell metabolism, as well as disease processes. We also develop transient absorption microscopy to study a wide range of red blood cell associated functional processes such as neurovascular coupling in mouse brain. Together, we aim to build an integrated chemical imaging platform and couple it with advanced data analysis for comprehensive structural and functional imaging of living biological samples at single cell resolution.
Professor Fu's research
Research in Professor Fu's group focuses on developing novel optical spectroscopy and imaging techniques to investigate the spatial-temporal dynamics of living biological systems at single cell resolution. In particular, researchers are interested in using label-free spectroscopic imaging approaches such as stimulated Raman scattering microscopy to study the cellular mechanism of complex diseases such as cancer and metabolic disorders. They draw on expertise from multiple disciplines including analytical chemistry, ultrafast spectroscopy, imaging science, optical engineering, bioinstrumentation, cell biology, and systems biology.