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Principal Research Interests

The focus of our research is to investigate the structural basis for carcinogenic and anticancer activity of DNA- and protein-modifying agents. Synthetic methodologies are developed to prepare structurally modified nucleosides and amino acids representing carcinogen- and drug-induced DNA and protein adducts. The effects of nucleobase modifications on DNA structure and stability are determined by NMR spectroscopy, mass spectrometry, CD spectroscopy, and computer modeling of chemically altered DNA. Biological mass spectrometry techniques are employed to quantify the formation of DNA and protein adducts in vivo. These studies identify the molecular targets of exogenous and endogenous electrophiles and provide an insight into the origins of their biological activity. The following projects are currently underway:

  • identification of DNA adducts produced by bis-electrophiles,
  • solid phase synthesis of DNA oligonucleotides containing site- and stereospecific DNA adducts,
  • inter-individual differences in metabolism of tobacco carcinogens,
  • DNA sequence effects on reactivity, and
  • novel mass spectrometry methodologies for quantitative analyses of DNA damage.

Adviser To

Mailing Address

  • Natalia Tretyakova, University of Minnesota, Department of Chemistry
  • CCRB (delivery code 2812), 139 Smith Hall, 207 Pleasant St SE
  • Minneapolis, MN 55455-0431